The objective of the study was to investigate the combination of carvacrol (CVL) and rosiglitazone (RSG) on non-enzymatic and enzymatic antioxidants in the plasma and tissues of high fat diet (HFD) induced diabetes in C57BL/6J mice weighing 20-30 g. The normal and diabetic mice were treated with CVL (20 mg/kg BW) dissolved in 0.5% dimethyl sulfoxide (DMSO) and RSG (4 mg/kg BW) dissolved in water for 35 days. Diabetic mice had an elevation in the levels of lipid peroxidation markers, thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides (LOOH) and conjugated dienes (CD) also a reduction in non-enzymatic antioxidants, vitamin C, vitamin E and reduced glutathione (GSH) in the plasma and tissues, and enzymatic antioxidants, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S- transferase in the erythrocyte and tissues. Treatment with CVL and RSG in combination brought the non-enzymatic and enzymatic antioxidants to near normality when compare to other groups.
