In order to promote increased efficiency of cultivation techniques for higher yield of culture in a sustainable manner, the objective of this study was to evaluate alternative sources of lettuce fertilization in the north of Mato Grosso. The cultivar Marisa was used to obtain answers regarding the agronomic performance and physical characteristics. The experimental design was a complete randomized block design with four replications. The treatments were: T1 - control (without fertilizer); T2 - chemical fertilizer (NPK); T3 - chicken manure; T4 - Sheep manure and T5 - Cotton waste (by-products of cotton trees). These were evaluated: shoot diameter, fresh matter (without root), dry matter, leaf length, number of leaves, daily increment and commercial productivity. The fertilization with animal manure and vegetable waste presented better results in relation to chemical fertilizer (NPK). The incorporation of sheep manure provides greater shoot diameter, fresh matter, dry matter, leaf length, daily increment and commercial productivity.