Antibiotic effect of ethanolic extract of seeds of coffea canephora by disc diffusion and determination of its minimum inhibitory concentration in selected strains of bacteria

Aim: The aim of this study was to compare and evaluate the antibacterial efficacy of alcoholic extract of coffe a canephora on periodontal pathogenic bacteria Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Fusobacterium nucleatum (Fn) and Aggregatibacter actinomycetemcomitans (Aa) with 0.2 % chlorhexidine gluconate and distilled water. Materials and methods: The alcoholic extract of coffea canephora was obtained using percolation technique with ethanol as a solvent. Minimum inhibitory concentrations (MICs) and disk diffusion test were used to assess the antibacterial activity of alcoholic extract of coffea canephora on periodontal pathogenic bacteria with 0.2% chlorhexidine gluconate as positive control and distilled water as negative control. Results: The disk diffusion test and the MIC showed that the coffea canephora extract showed antibacterial activity against the four periodontal pathogens which is comparable to chlorhexidine. Conclusion: Alcoholic coffea canephora extract possess good antibacterial activity against Aa, Pg, Pi and Fn and thus would be beneficial for the prevention and treatment of periodontal disease.