Studies suggest that the autolysis of bacterial strains also enhances the biofilm formation and the increase in incidence of pathogenicity. When the strains are subjected to adverse conditions either acidic or alkaline or to stress tolerance or to heat pressure, the parameters described above are all affected adversely. During this period, as a matter of escapism or to gain resistance power, the bacteria undergo autolysis. The pathogenecity of the organism tries to increase and also makes sure that its genomic DNA is released into the external surroundings. Pure river bodies are being contaminated by anthropogenic methods. Their contamination makes the water switch to acidic or alkaline environment. Depending on the source of contaminants the water becomes either highly acidic or highly alkaline. This fluctuation not only kills the flora and fauna of the ecosystem, but also adversely affects the pathogenicity of the bacterial species. When bacteria are subjected to adverse condition (pH), it undergoes autolysis as a mode of protection, survival and tolerance. Studies also suggest that the autolysis of bacterial strains also enhances the biofilm formation and the increase in incidence of pathogenicity. For the first time we try to find out the possible cooperative role of autolysis and biofilm formation and its antibiotic resistance power. The results confirmed of the presence of autolysis during adverse conditions. Our hypothesis of the pathogenic strains escaping from the unfavourable conditions by increasing its antibiotic resistance is a well marked figure in our thesis. The results also clearly showed that, the strains showed an enhancement in the formation of the biofilms.
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