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Antioxidant activity and phenolic profiles with hplc of Consolida orientalis

Author: 
Seyda Akkaya, Merve Badem, SilaOzlem Sener, Nuriye Korkmaz and Rezzan Aliyazicioglu
Subject Area: 
Health Sciences
Abstract: 

Background and Objectives: Consolida genus belongs to Ranunculaceae and includes about 52 species world-wide. The aim of our study was to investigate in terms of phenolic contents and antioxidant capacity of Consolida orientalis. Materials and Methods: Reverse phase-high performance liquid chromatography (RP-HPLC) was used for the identification of the phenolic compounds of C.orientalis. Antioxidant activity of methanolic extract was investigated by two methods, namely ferric reducing antioxidant power (FRAP), and 2,2-diphenylpicrylhydrazyl radical scavenging (DPPH) activity. Results: Chlorogenic acid, p-hydroxy benzoic acid, caffeic acid, p-coumaric acid, and sinapic acid were determined as main phenolic compounds in the methanolic extract. The IC50 value for DPPH assay has been found as 0.4178 ± 0.0113 (mg/mL), FRAP value is 200 ± 1.732 (μMTrolox/g sample), and total phenolic content value is 6.6 ± 0.153 mg gallic acid per gram sample in methanolic extract of the aerial parts of C.orientalis. Conclusion: The methanolic extract of C.orientalis might be used as raw material by pharmaceutical and food industries for the preparation of natural drugs.

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