The antibacterial activity of extract of medicinal plants, namely, Rhizophora apiculata, Phyllanthus emblica, Avicennia marina, Acalypha indica and Withania somnifera was evaluated against urinary tract infectious pathogens Staphylococcus aureus, Klebsiella pneumoniae, Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli. As compared to ethanol, acetone extract showed broad-spectrum activity. The multidrug-resistant (MDR) bacteria Enterococcus faecalis was inhibited by the acetone extract of Phyllanthus emblica fruit whereas the other two resistant bacteria Staphylococcus aureus and Escherichia coli were inhibited by both ethanol and acetone extract of all the species. Biochemical analysis revealed the presence and confirmation of the organism. Further studies using different solvents for extraction are necessary to confirm that medicinal plants are a better source for the development of novel antibiotics.