Data on biosecurity measures were gathered from Ministry of Agriculture and Animal Resources (MAAR), Khartoum State, Sudan with the objective to evaluate biosecurity status in poultry industry in Khartoum state. The data referred to one year work executed by the veterinary authorities during 2013 with the objective of measuring biosecurity level for registration purposes. The data were organized in twelve biosecurity variables prior analysis. The total number of farms visited was 59 of which 16, 33 and 10 were characterized as traditional, modern and semi modern production systems, respectively. The results showed that modern and semi modern farms are located at Khartoum and Bahri provinces and that the percent of broiler farms (69.5%) was found to be greater than that of layers farms (30.5%). Broiler industry is dominated by modern and semi modern systems. The survey results indicated that there was an overall low frequency of adoption of biosecurity measures (P = 0.01) by the poultry growers in semi modern and traditional systems. However, biosecurity measures were higher in modern production system although the compliance with biosecurity measures was not uniform among all farms in this system (P = 0.01). The results also found that poultry farmers have laid more consideration on those biosecurity measures targeting dead birds' disposal, veterinary supervision and vaccination variables.