Differents characterization of lactic acid bacteria isolated from adiopodoume’s farms in ivory coast

Isolation of lactic acid bacteria (LAB) from poultry suburbs farms of Adiopodoumé-Abidjan (Ivory Coast) was carried out. LAB were cultivated on lactic acid medium (MRS) and were characterized based on colonies morphology, cells morphology and biochemical tests. Lactic acid bacterial strains were isolated from soils, chicken faeces and feathers.Out of fifteen samples analysed, thirteen (86.6%) harboured LAB. All isolates were obtained by sequential screening for catalase activity and Gram-staining.Out of (86,6%) which five (33,3%) were established to be homofermentative by the gel plug test. Four isolates (26,6%) were identified by use of the API 50CHL kit and threeLactobacilli strains and one Lactococci (0,066%) strain were selected to study their growth and lactic acid production profiles in a time course experiment. The Lactobacilli strains, both isolated from faeces and fresh intestine, produced higher amounts of cells and lactic acid from soils as compared to theLactococci strain isolated from feathers. L(+)-lactic acid is the only optical isomer for use in pharmaceutical and food industries because is only adapted to assimilate this form. The opticals isomers of lactic acid were examined by L(+) and D(-)-lactate dehydrogenase kit. Lactobacilli strains produced combination of both optical isomers of lactic acid. Among them, Lactobacillus casei subsp. casei produced the low amount of D(-)-lactic (2%). The optimum rates of glucose for lactic acid production by Lactobacillus strains were 180 and 120 g/L for Lactobacillus plantarum and Lactobacillus paraplantarum, respectively.