Back ground: In most invertebrate systems, dietary restriction is applied somewhat non-traditionally in that food quality, rather than quantity, is manipulated through dilution of the nutritional components in the medium. To clarify how these nutritional factors influence longevity, nutrient consumption and lifespan, we were measured on a series of diets with varying glucose and vitamin C content. Methods: The fly stock is assessed for this experiment is Drosophila melanogaster. The stock is obtained from the Drosophila stock centre, Mysore, India. For the dietary restriction assays, different concentrations of Glucose (3g/100ml, 5g/100ml and 7g/100ml), vitamin C (0.25mg/100ml, 0.5mg/100ml and 0.75mg/100ml) and both Glucose plus vitamin are formulated in the diet. For longevity analyses, we used the modified protocol of Luckinbill and Clare, 1985. Results: The flies fed with combination of Glucose plus Vitamin C resulted in an increased life span followed by Vitamin C and Glucose in both males and females when compared to control. However LD of Glucose fed flies have resulted significantly decreased lifespan. Interestingly female flies have shown significantly increased life span in all the concentrations. Discussion: In several studies has been demonstrated that increased intake of protein may increase protein synthesis, decrease protein breakdown, reduce fat accumulation, and increase fat-free mass. The flies fed with combination of Glucose plus Vitamin C resulted in an increased life span followed by Vitamin C and Glucose in both males and females when compared to control.
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