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Effect of addition of antioxidants in the freezing of boar semen on the motility and viability of sperm

Author: 
Alejandro Córdova Izquiedo, Adrian Emmanuel Iglesias Reyes, Román Espinosa Cervantes, Juan Eulogio Guerra Liera, Jorge Fabio Insunza Castro, Abel Edmundo Villa Mancera, Maximino Méndez Mendoza, Rubén Huerta Crispín, María de Lourdes Juárez Mosqueda, Arman
Subject Area: 
Health Sciences
Abstract: 

The aim of this study was to assess the effect on the use of vitamin C, E and C + E as antioxidants on sperm quality of frozen-thawed semen boar semen Yorkshire breed, which was divided into three treatments with Vitamin C, E and combined (C + E) at different concentrations (0, 2, 4 and 6 mg / ml) was used. Motility and viability was assessed before and after freezing the semen, semen freezing was described by Westendorf based with some modifications. After thawing with vitamin C motility of 80% and viability of 75%, with vitamin E motility was obtained was 85% and a viability of 89% and vitamin C + E motility was 85% and 88% viability. Concentration of 4 mg / ml in the control treatment vitamin C was 81% and 85% viability, with E motility was 80% and 85% viability. With vitamins C + E motility was 86% and 88% viability before freezing. After thawing, motility vitamin C was 80% and 80% viability; with E motility was 80% and 83% viability; with C + E vitamins in the witness vitamin C motility was 83% and the viability of 85%: In the concentration at 6 mg / ml the witness with vitamin C motility was 81% and viability of 80% ; with E motility was 87% and the viability of 80% and vitamins C + E motility was 85% and the viability of 88%: After thawing, the C motility was 83% and 79% viability, with E motility was 85% and 83% viability with vitamins C + E motility was 87% and 88% viability. In conclusion, the addition of vitamins in the spermatic conservation help keep sperm motility and viability.

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