Due to wide industrial use, chromium and copper are considered as serious environmental pollutants. Hexavalent chromium is one of the heavy metal and it was found to cause variety of clinical problems like asthma, pneumonitis, bronchogenic carcinoma, skin allergies and so on. Cu toxicity also causes problems like abdominal pain, nausea, vomiting, headache, diarrhoea, respiratory difficulties, anaemia, gastrointestinal bleeding, kidney failure and death. Plants are becoming more efficient producers of food, fiber and medicines. Apart from these conventional uses, biotechnology unlocks the doors to unique uses of plants that are gaining greater acceptance and attention from the people and the scientific community. These are called ‘‘value-added’’ uses include phytoremediation and hence the objective of the present study is to explore the full potential of plant tissue culture techniques to study the metal tolerance in whole plant in culture. The model plant system used in this study is a cultivated variety of mustard, groundnut and the metal that had been used is Chromium and Copper in six different concentrations.