Studies were conducted to assess the effect of different organic additives on the production of lignocellulolytic enzymes and their influence on the spawn and sporophore production. Horsegram flour influenced the maximum production of Exo -1,4 glucanase, Endo -1,4 glucanase, -glucosidase, Laccase, Xylanase and Polyphenol oxidase in Hypsizygus ulmarius. Among the different spawn substrate paddy grain recorded the fastest mycelia growth covering the spawn bottle in 12.05 days. Mushroom beds inoculated with Paddy grain spawn recorded maximum number of sporophores (118.00), maximum weight of sporophores (492.88 g bed-1) and the best biological efficiency (98.57 %). Among the different additives viz., Blackgram flour, Cotton seed Powder, Finger millet flour, Groundnut cake, Horsegram flour, linseed cake, Redgram flour and sorghum flour. Horsegram flour supplemented paddy grain recorded minimum spawn run days (10.1 days) and subsequently inoculation of spawn supplemented with horsegram provided highest biological efficiency (103.01%) of Hypsizygus ulmarius.