Efficient plant regeneration via organogenesis in spilanthes acmella m. An important medicinal plant

Micropropagation and organogenesis successfully achieved in Spilanthes acmella M. is belongs to family Asteraceae and commonly known as Akarkara. Multiple shoots induced from nodal explants on Murashinge and Skoog (MS) medium supplement with various growth regulators BA and Kin alone. The highest shoot regeneration frequency was recorded on MS medium supplement with 5 μM BA (97.3 ± 1.2%), with highest number of multiple shoots (12.2 ± 0.9). In vitro raised shoots were excised and implanted on MS half strength medium supplement with various concentrations of auxins (NAA and IAA, 1.25 – 15 µM) to produce roots. The maximum frequency of roots obtained on MS medium fortified with 5 µM IAA (31.40 ± 3.2). The regenerated plantlets were successfully transferred into the pots containing sterilized soil and sand (3:1) combination.