
This study attempted to monitored the effect of various parameters which effect the bioelectricity generation by the nano-wire forming electrogen Proteus sp. BVB09 isolated from the cow dung in two chambers MFC having cellophane membrane as PEM. Experimental results concluded that mineral media M6 produced highest bioelectricity of 988.32mW/m2 (397.8mV, 0.004mA, and 2.48mA/m2) on the 6th day of the MFC operation at highest external resistance of 100KΩ whilst on the optimization of the necessary factors, a significant bioelectricity generation of 244363.97mW/m2 (198.70mV, 1.98mA and 1229.81mA/m2) on the 26th day of the MFC operation at 100Ω was recorded. These results obtained were also directly proportional to the bio film and biomass accumulated at the anode and anolyte of the MFCs.