Many conservation measures aim the control of poaching and new threats to wildlife in India; but on the other hand, many infectious diseases and clinical problems taking upper hand in many of the wild animals are always given less significance than they actually deserve. Considering all these, in order to add more essence to the conservation biology, the present work may act as a tool for successful conservation in India. Water samples from Mudumalai Wildlife Sanctuary, Anamalai Wildlife Sanctuary and forest divisions of Sathyamangalam-Erode regions were collected in a 250 ml sterile, clear air tight container and sealed using parafilm and tests were carried out in the laboratory. Results obtained on 24 and 48 hours incubation at 37οc were compiled. The present research paper communicates the evidence of coliforms in the water samples collected from the Free-Raging Wildlife regions of Tamilnadu state during dry seasons of February- June, 2010.