The present study aims to evaluate a relationship between land-use and two water quality parameters i.e., nitrate and total dissolved solids (TDS) for selected areas within Karachi city of Pakistan during the year 2014. The water pollution data was combined with satellite land-use coverage in a Geographical Information System (GIS)-interface using Arc view 3.2 followed by Pearson’s correlation (statistical) analysis. The study remained highly successful in predicting drinking water pollution source. The nutrient loading in drinking water was positively correlated with vegetation land-use and negatively correlated with undeveloped land.