Urinary Tract Infection (UTI) is one of the most common infectious disease ranking next to upper respiratory tract infection is the cause of morbidity and mortality in human. They are mostly caused by bacteria. 50-80% women experience UTI at least once or twice in their lives. Enteric pathogens (e.g. E.coli.) are most commonly responsible, it is well established that for UTI, but Klebsiella sp., Enterobacter sp. and Pseudomonas aeruginosa, Proteus sp. are also responsible grams positive organisms including Enterococcus sp. Staphylococci and Streptococci have also been found to cause severe infections in human being. Therefore, studying and identifying bacterial pathogens causing UTI through biochemical test is the highest priorty.