Immobilization studies on multi-enzymes produced by a soil isolate aeromonas jandaei

The present study was carried out to immobilize the multi-enzymes produced by a soil isolate Aeromonas jandaei. The three enzymes viz. amylase, lipase and protease produced by this isolate were partially purified by dialysis and were immobilized using 3 matrices- agar, agarose and alginate at 3 different concentrations. A washout of enzymes was seen at the lower concentrations of the matrices. At 3 % concentration the enzyme substrate interaction was reduced as low activity was seen. 2 % agar and agarose gave excellent enzyme retention and activity. 1 and 2 % alginate beads however dissolved in the reaction medium; the enzyme thus immobilized could not be retained for reuse. However 3 % alginate did not dissolve and was retrievable. 2 % agar was said to be the best choice for immobilization as it is cheaper than agarose and can be reused. Also when the enzyme is not to be reused and requires to be released into the reaction medium, alginate could be a preferable choice and can be used as a delivery/ transport medium for the enzymes.