Background: The stability of biomarkers in stored biomedical samples is crucial, especially when storage is for extended periods of time. High-sensitivity CRP (Hs-CRP) is a biomarker of low grade inflammation that is extensively used to identify and study card in vascular and/or inflammatory processes in clinical care and large epidemiologic studies. (Ayo et al., 2014) Therefore, assessing Hs-CRP stability in archived samples at a given temperature is important to insure precision of measurements over time and the validity of studies using archived samples. Methods: We evaluated the stability of Hs-CRP in 30 randomly selected human serum samples by measuring Hs-CRP concentrations in freshly collected sample (Hs-CRP (0)) and in the same set of samples after 7–11 years of storage at −80°C (Hs-CRP (LT)). Results: Hs-CRP did not significantly change up to 11 years of storage at −80°C as shown by a negligible median difference between Hs-CRP (0) and Hs-CRP (LT), delta(Hs-CRP (0)- Hs-CRP (LT)=−0.01, p=0.45. There was a good concordance and agreement between Hs-CRP (0) and Hs-CRP (LT) as measured respectively by Lin's coefficient of correlation (ρC= 0.98) and Bland-Altman analysis (mean difference = −0.02, 95% CI (−0.04–0.0045) p=0.107). In addition, the data also suggest that the time elapsed between collection and Hs-CRP measurement does not affect Hs-CRP stability over time when samples are kept under the appropriate conditions. Conclusions: Long-term storage at −80°C for up to 11 years did not significantly affect the stability of serum Hs-CRP. Given the cost and time for collecting fresh samples, this observation represents an important finding for biomedical research and clinical care.
IJCR is following an instant policy on rejection those received papers with plagiarism rate of more than 20%. So, All of authors and contributors must check their papers before submission to making assurance of following our anti-plagiarism policies.
