This study was designed to determine the incidence of H.pylori in different types of food and water in Dhamar Governorate. Four hundred and twenty six samples (426) were collected included 114 Red meat, 100 Poultry meat, 100 Salad and 112 Water. The results revealed that the isolation rate of H.pylori from total samples was (11.7%), the highest rate of H.pylori isolation were found in red meat samples (15.8%), then poultry meat (13.0 %), water (11.6%), and salad (6.0 %). When we study the relationship between prevalence of H.pylori in food and water with months during the period of study. April, June and May take the more isolated rate (24.5%), (20.4%) and (16.4%) respectively, while the rate of isolation were seen decrease whenever moved away from these three months and in both directions. The results indicated that there is significant differences at (p < 0.05) between isolation rate of H.pylori from above samples and months. From this study we concluded that the incidence of H.pylori in food and water in Dhamar Governorate was high, also found that food and water have a significant role in transmission of H.pylori to human.