Isolation and identification of soil Microflora in the rice fields of Narasannapeta mandal in Srikakulam district

Soil mycoflora were isolated from the rice fields of Narasanapeta during a period of one year ie., from January 2013 to December 2013. Isolation of the mycoflora was done by serial Dilution Plate Method. During the investigation period a total of 118 fungal colonies of 27 fungal species were observed. Identification was done by using standard manuals and literature. The maximum percentage contribution was that of Aspergillus niger (13.55%), followed by Aspergillus flavus (11.01%) and the minimum percentage contribution was that of Aspergillus restrictus and Trichoderma koeningii (0.84%) and also of unidentified species of Mucor and Penicillium. Among the isolates were Aspergillus candidus, A.clavatus, A.flaviceps, A.flavus, A.fumigatus, A.nidulans, A.niger, A.oryzae, A.restrictus, A.tamarii, A.terreus, Bispora species, Curvularia clavata, C.lunata, Fusarium oxysporum, F.solani, Mucor recimosus, Mucor species,Penicillium chrysogenum, P.frequentens, P.funiculosum, P.species, Rhizopus nigricans, R.stolonifer, Trichoderma harzianum, T.koeningii and T.viride were authentically characterized. Most of the fungal species belonged to anamorphic fungi ie., Deuteromycotina (94 colonies), Zygomycotina (17 colonies) and the remaining to that of unidentified white mycelia (7 colonies) were observed. The percentage frequency of the fungal species was statistically analyzed.