Neuroprotective effect of methanolic leaf extract fractions of butea monosperma on β-amyloid peptide induced amnesia in mice

The objective of this investigation was to evaluate the neuroprotective activity of methanolic leaf extract fractions (ethyl acetate, n-hexane, chloroform and aqueous) of Butea monosperma (BM) on β-amyloid peptide induced amnesia in mice. From previous studies, the methanolic leaf extract showed neuroprotecive activity in amnesia mice. Further, the leaf extract is subjected to fractionization by using ethyl acetate, n-Hexane, chloroform and water as solvents. The fractions are subjected to jumping test and Y-maze test, rectangular maze test and also for biochemical parameters such as acetyl cholinesterase enzyme (AChE), peroxidase, MDA and DPPH radical scavenging activity. From the results, it was found that the fractions of extract possess neuroprotecive activity and ethyl acetate fraction having significant anti-amnesic activity and anti oxidant activity compared with other three fractions. By identifying and isolating the chemical constituents responsible for anti-amnesic and anti-oxidant activities, we may find a breakthrough in the treatment of other neurodegenerative disorders which occur due to oxidative stress.