Meyna spinosa Roxb, a medicinal plant enjoys it use in the traditional medicine in all over the India for the treatment of a number of ailments. In Bengali it is called as Monkata. It is a wild common plant distributed in India, Bangladesh and in both northeastern tropical Africa and tropical Asia. The main objective of the present research work was to determine various bioactive compounds and to evaluate the in vitro antibacterial activity of different fruit extracts of Meyna spinosa Roxb against different gram positive and gram negative bacteria. Based on this, a new series of constituents had been planned to extract by Ethanol (E1), Methanol (E2), Ethylacetoacetate (E3) and Chloroform (E4) from the fruits of Meyna spinosa Roxb. The in-vitro antibacterial activity was carried out by Paper disc diffusion method and MIC was determined by Agar streak dilution method. The results displayed that the various extracts of fruits of Meyna spinosa Roxb, had a potential antibacterial activity against different gram positive and gram negative bacteria with an MIC range of 9-22 µg/ml (gram positive organisms) and with an MIC range of 10-26 µg/ml (gram negative organisms). The MIC values of various extracts (E1, E2, E3 and E4) of fruits of Meyna spinosa Roxb for different gram positive bacteria were found to be S. aureus (MIC: 9-18 µg /ml ), B.subtilis (MIC: 10-21 µg /ml), S. typhi (MIC: 11-22 µg /ml) and S. epidermidis ( MIC:12-21 µg /ml). The extracts (E1, E2, E3 and E4) of fruits of Meyna spinosa Roxb were active against all the tested gram negative microorganisms with the range of MIC values for P. aeruginosa (MIC: 10-20 µg/ml), P. flurocense (MIC:12-25 µg/ml) and E. coli ( MIC: 11-26 µg/ml) and V. cholerae (MIC:12-23 µg/ml).