Gluconacetobacter diazotrophicus, a true fruitful, sugar loving and acid producing endophytic diazotroph involved in N fixation, P solubilization, zinc solubilization and growth promoting substance production. It can also able to control fungal pathogens likes fusarium and Colletotrichum falcatum and helps the sugarcane plants to produce its maximum growth with the reduction of 50 per cent chemical fertilizers. The present field experiment was laid out with the following treatments. T0 -Absolute control, T1 - G. diazotrophicus alone, T2 - 50% NPK alone, T3 - 50% NPK + G. diazotrophicus, T4 - 75% NPK alone, T5 - 75% NPK + G. diazotrophicus, T6 - 100% NPK alone, T7 - 100% NPK + G. diazotrophicus and three replications were also maintained in the field studies. Among the eight treatments, the plants receiving G. diazotrophicus inoculation showed the vigour in the growth and developments of sugarcane, particularly the treatments. T3 - 50% NPK + G. diazotrophicus and T5 - 75% NPK + G. diazotrophicus recorded the growth values on par with the growth values of plants receiving 100% NPK + G. diazotrophicus (T7) and T6 - 100% NPK alone. Hence, by using G. diazotrophicus we can able to reduce 50% usage of chemical fertilizers and it leads to reduce the pollution to some extent in sugarcane cultivating soil and environment.