Studies on membrane permeability, lipid Peroxidation activity and SDS-page for improved Germinability and field performance of Invigourated high medium Vigour soybean seed

Five-month old stored soybean seeds (Glycine max [L.]Merr., cv. Soyamax) were subjected to dry and wet treatments. Dry treatments were done with powdered crude plant materials viz. red chilli powder @1g/kg of seed, neem leaf powder @2g/kg of seed, chemicals viz. iodinated calcium carbonate @ 2g/kg of seed (30mg iodine impregnated to 2g calcium carbonate), para-amino-benzoic acid @500mg/kg of seed, ferulic acid @500mg/kg and pharmaceuticals viz. aspirin @50mg/kg of seed. In case of wet treatments, Soaking-Drying (S-D), Moist-Sand-Conditioning-Drying (MSC-D), Moist-Sand-Conditioning-Soaking-Drying (MSC-SD) were employed with dry treatments. The results revealed that, wet treatment, MSC-SD followed by dry treatments with aspirin and neem leaf powder significantly improved the germination percentage, total seedling length, vigour index, field performance and productivity. Soaking-drying showed adverse effect on germinability probably due to soaking injury. Physiological and Biochemical studies indicate that MSC-SD followed by aspirin, neem leaf powder and iodinated calcium carbonate showed reduced leakage of electrolytes, sugars, amino acid with lower lipid peroxide formation than the control. It has also been noted in SDS-PAGE analysis that the invigorated seeds, especially MSC-SD showed higher number of polypeptide bands with high banding intensity than the control. On the basis of the observation, MSC-SD may be recommended for the improvement of storability as well as field performance and productivity of high medium vigour soybean seeds.