The success of establishing new plantations of a perennial crop like coffee mainly depends on the use of healthy seedlings with good vigour for planting. For this purpose, it is most essential to protect the seedlings in the primary and secondary nursery beds from attacks of pest and diseases. Coffee seedlings at the nursery stage are susceptible to fungal diseases. In the persent study four Trichoderma spp were tested for their ability to inhibit soil borne pathogen Rhizoctonia solani Kuhn. causing collar rot in coffee seedlings. Dual culture technique revealed that T. hamatum (75.68%) highly inhibited mycelial growth of pathogen followed by T.koningii (65.92), T.viride (57.90%) and T.harzianum (52.71%) respectively. This preliminary results are valuable for further studies.