Bioethanol is one of the energy sources that can be produced by renewable sources. Waste potatoes relatively inexpensive as compared with other feedstock considered as food sources. However, a pretreatment process is needed. In Chitrakoot a much amount of potatoes are produced every year. This research was carried out to find out the optimum conditions for bioethanol production. The protocol was used by co-cultures of Saccharomyces cerevisiae and Aspergillus niger under simultaneous saccharification and fermentation (SSF). Bioethanol production was done at different temperature (25 to 35°C), pH (4 to 6) and fermentation protocol was done at 96, 120, 144 hours. The optimal condition for the production of bioethanol was 6 pH and 30°C temperature. The fermentation of potatoes was the highest at 144 hours with yeast concentration 4 to 6 %.The maximum ethanol yield from waste potatoes was 11.14% and amount of ethanol content increased with the increase in fermentation time.