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Acetyl Choline Esterase Activity In Muscle Tissue Of Epileptic Albino Mice

Author: 
Maleeka Begum, S. F., Srinivasan, J., Chandrasekar, S., Savitha, C. and Preethy Mani, M.
Subject Area: 
Life Sciences
Abstract: 

Albino mice weighing approximately 60±0.5 gm were taken as experimental and control animals. Both the control and experimental mice were injected intraperitonally with atropine to reduce peripheral activity. Pilocarpine was dosed along with phosphate buffer against the muscarinic and glutamate receptors to the experimental mice to induce seizures in the mice. Seventeen days after the injection, the experimental and control mice were sacrificed. The brain parts and other body parts were dissected. The muscle of the experimental and control mice were taken for the experiment. Acetyl choline esterase (AchE) assay was done in muscle tissue of epileptic mice by spectrophotometric method. The kinetic parameter Vmax, the maximum rate of reaction and Km, Michaelis menten constant of acetyl choline esterase enzyme were studied in the membrane fraction of muscle tissue in epileptic condition. The result shows increase in the Vmax during epileptic condition than that of the control. Michaelis Menten constant was found to be decreased.

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