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Anti-cancer activity in hepatocellular carcinoma cell line hepg2 from folklore plant

Author: 
Veerabhadrappa, K. V., Raveendra Reddy, J., Padmanabha Reddy, Y., Narayana, G., Pradeep Kumar, B., Haranath, C., Sanjeeva Kumar, A. and Sudir, A.
Subject Area: 
Health Sciences
Abstract: 

Hepatocellular carcinoma (HCC) is a primary malignancy of the liver and occurs predominantly in patients with chronic liver disease and cirrhosis. A better knowledge of molecular Hepato carcinogenesis provides today the opportunity for targeted therapy. Most of the existing anti-cancer produces side effects such as fever, hallucinations, low blood pressure, bone marrow problems, paralysis, coma, liver problems, and kidney problems. The present study aimed. The herbal extract were prepared in solvents like ethanol and screened for ant cancer activity. MTT assay is a colorimetric assay used for the determination of cell proliferation and cytotoxicity, based on reduction of the yellow colored water soluble tetrazolium dye MTT to formazan crystals. Mitochondrial lactate dehydrogenase produced by live cells reduces MTT to insoluble formazan crystals, which upon dissolution into an appropriate solvent exhibits purple color, the intensity of which is proportional to the number of viable cells and can be measured Spectro- photo metrically at 570nm. Viability of cell decreasing with increasing dose and 450 ug/ml of the extract shows 60% viability count and shows good anticancer activity compared to standard drug, Aim: The present study was aimed to investigate Pharmacognostical, Phytochemical screening and Anti-cancer activity in hepatocellular carcinoma cell line HepG2 from folklore plant Materials and Methods Assay controls: • Medium control (medium without cells) • Negative control (medium with cells but without the experimental drug/compound) • Control (medium with cells treated with a known drug, Metformin; 5uM) HepG2 200 μl cell suspension without the test agent. Allow the cells to adhere to the culture plate for about 24 hours. Add test agent incubate 37°Cin a 5% CO2 atmosphere. Add MTT reagent incubates for 3 hours. Then add solubilisation solution (DMSO) in an amount equal to the culture volume. Gentle stirring in a gyratory shaker will enhance dissolution. Occasionally, pipetting up and down may be required to completely dissolve the MTT formazan crystals especially in dense cultures. Read the absorbance on a spectrophotometer or an ELISA reader at 570nm. Results and Conclusion: Folk plant extract after treated with HepG2 cell Viability decreasing with increasing dose and 450 ug/ml of the extract shows 50% viability count and shows good anticancer activity compared to standard drug.

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