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Antibacterial activity of 10% carbamide peroxide bleaching agents on periodontal pathogens” – an in vitro study

Author: 
Dr. Shamila Shetty, Dr. Megha Vanasi, Dr. Nandini Manjunath, Dr. Nidha Begum and Dr. Thasneem, A.A
Subject Area: 
Health Sciences
Abstract: 

Aim: The aim of this study was to compare and evaluate the antibacterial activity of commercially available 10% carbamide peroxide bleaching agents on periodontal pathogenic bacteria with 0.2% chlorhexidine gluconate. Material & Methods: Two commercially available bleaching materials containing 10% Carbamide peroxide were selected for the study against Chlorhexidine solution (0.2%). Different bacterial stock were used. Wells each with a diameter of 5 mm were punched into each of the agar plates and filled with either one of the commercial bleaching materials or with Chlorhexidine solution (0.2%) to test for susceptibility test. All plates were incubated for 24 to 48 h at 37°C in a candle jar. The diameter of each zone of inhibition was measured, and the mean was calculated. Results: Susceptibility tests were performed on disk sensitivity (DST) agar used routinely for antibiogram tests showed that Chlorhexidine group showed the highest mean value of Prevotella intermedia (23.67) and least in Actinobacillus actinomycetem comitans (20.33) with p value of 0.869. Opalescence Group using one way ANOVA test showed the mean value of Fusobacterium nucleatum (18.33) and least in Porphyromonas gingivalis with test value of 3.777 and p value of 0.027. Dr.collins group showed the highest mean value of Fusobacterium nucleatum (12.67) and no against Prevotellaintermedia (0) with a test value of 169.7 and p value of <0.001. Conclusion: Chlorhexidine (0.2%) displayed higher antibacterial effects on the periodontal pathogens with the highest mean value against porphyromonas gingivalis at 23.67 than both the tested bleaching materials containing 10% carbamide peroxide in this in-vitro study showing that chlorhexidine would thus be more beneficial for the prevention of periodontal diseases.

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