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Antibacterial efficacy of clindamycin gel - incorporated obturating materials for primary teeth against enterococcus faecalis – an in - vitro study

Author: 
Dr. Savitha Sathyaprasad and Dr. Devalaraju Meghana
Subject Area: 
Health Sciences
Abstract: 

Aim and objective: To assess the Antibacterial efficacy of clindamycin gel incorporated Zinc oxide eugenol and Endoflas FS, two obturating materials used in pulpectomy, against enterococcus faecalis in primary teeth. Materials and methods: The study consisted of four groups, with 11 samples in each group: group I (zinc oxide eugenol paste), group Ia (Zinc oxide eugenol paste + 2% Clindamycin gel ), group II (Endoflas FS), and group IIa (Endoflas FS+ 2% clindamycin gel). A double layer agar well diffusion test was used to evaluate the antibacterial activity against enterococcus faecalis. The zones of microbial inhibition were measured at the end of 24 hours, 6th day, and 29th day. Results: On intergroup comparison, the difference in the antibacterial activity was found to be highly significant (p <0.001). Among the various groups evaluated, group IIa showed the highest antibacterial activity against E. faecalis followed by group II, group Ia, and the least activity being shown by group I throughout the experimental periods. On intragroup comparison at different time intervals, a maximum zone of inhibition was seen at 24 hours with a p value < 0.05 in all the tested groups. Conclusion: Incorporation of 2 % Clindamycin gel into Zinc oxide eugenol and Endoflas FS enhanced the antimicrobial activity of both the root canal filling materials with lasting antimicrobial activity even at the end of the 29th day. Clinical significance: An essential prerequisite for a root canal filling material is its antibacterial efficiency, which will aid in eliminating residual microorganisms in the root canal system following chemo mechanical preparation. A pulpectomy-treated tooth has a better prognosis when the root canal filling materials' antibacterial efficiency is greatly increased when an antimicrobial agent, like clindamycin gel, is added to them.

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