Citrus is amongst the world's most frequently farmed commercial fruit crops, and it is constantly exposed to a variety of environmental constraints including abiotic and biotic stresses. Since citrus plants have a very few and short root hairs. So, in order to get sufficient nutrient and water they need mycorrhizal colonization. Numerous soil microbes, particularly arbuscular mycorrhizal fungi, dwell into the citrus rhizosphere and form a mutualistic relationship with citrus plant roots. AMF has been considered as a valuable biofertilizer for sustainable agriculture since it provides resistance to host plants against environmental challenges. Moreover, mycorrhizal hyphae contribute towards soil aggregation wich ultimately increases the soil fertility. Although, AMF possess a broad array of applications in citrus plant performance; however, the molecular regulatory mechanisms underlying the AMF response have not yet been fully characterized in citrus plants. In this review, we aimed to dissect the intricate molecular and metabolic pathways induced within citrus following AMF colonization using a variety of approaches such as transcriptomics, proteomics and metabolomics. And further scrutinized the relative contributions of diverse processes to the modulation of host defense response. The insights provided from a more comprehensive understanding of this peculiar symbiotic relationship will contribute in the agricultural biotechnology advancements.