Aim: To assess the level of knowledge on biomedical waste management among staff nurses in selected tertiary hospital, Kanchipuram district, Tamilnadu. Methods and Materials: A descriptive approach was used for the present study. The participants were about 60 in number and purposive sampling technique was used. Data was collected by administering a structured questionnaire. Results: The study result shows that majority (90%) of staffs belongs to 21-30 Age group. A majority (87%) of the staffs were female and 60% of the staffs were belongs B.sc nursing. Majority (60%) of staff were underwent continuous nursing education programme on biomedical waste management. The study result reveals that 60% of staff nurse had moderate knowledge, 33% had adequate knowledge and 7% had inadequate knowledge. The result shows that the age (x2=6.666) had significant association with the level of knowledge at 0.05 levels and also the CNE programme attended (x2=13.262) had significant association with the level of knowledge. Conclusion: This result insists the importance of continuing education through skill based training programme and updating current changes in bio medical waste management for staff nurses.