Biofilmforming-pseudomonas aeruginosa isolated from burn patients in basra city, south of Iraq

Aims: 1- To evaluate the incidence of Pseudomonas aeruginosa bacterial pathogen in burn patients. 2- To determine the Biofilm producing P. aeruginosa Methods: Total of 62 samples have been collected from patients who attended to the center of Burn in Al-Faihaa General Hospital in Basra City from the period 01/03/2015 to 1/6/2015. Including 36 (58%) females and 26 (42%) males, aged between 6 months – 78 years. All specimens were directly inoculated onto plated of P.P.A and incubated at 37 ºC for 24 hrs and identified by microscopic examination and biochemical tests include (Oxidase, indole, Vogas-Proskauer, urease, Methyl red, citrate utilization and Kligler) and using CHROMagar media. Ability of P. aeroginosa to forming biofilm tested by using Congo Red Agar and confirmed by genetic detection using pslA, IcaA and IcaD genes by PCR technique. Results: The current results showed that, forty two (67.7%) positive samples were identified as P. aeroginosa by biochemical tests and CHROMagar medium, three samples (7.1%) were positive for biofilm forming on Congo red agar, three samples (7.1%) were positive to pslA gene, one (2.4%) positive to IcaA gene and all negative to IcaD gene. Conclusions: We wish to emphasize that the P. aeroginosa present at high frequency among burn patients, and this give difficult to healing infections. A screening procedure using CHROMagar media give better results and faster in identifying P. aeroginosa than conventional methods. Gene identification was the best biofilm forming test.