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Biogenic Synthesis, Characterization and Antibacterial Effect of TiO2 Nanoparticles

Author: 
Tirthesh Kumar Sharma, Vijay Kumar Yadav, Mukesh Shrivastav, Sippy Dassani, Ramendra Singh and Panday, M.M.
Subject Area: 
Life Sciences
Abstract: 

In recent years, nanotechnology has been explosion. Nano-structured materials are attracting a great deal of attention because of their potential for achieving specific processes and selectivity, especially in biological and pharmaceutical applications. Nanotechnology has become one of the most practical technologies, because of unique physical and chemical properties of nanomaterials. Our studies discuss recent advances in the synthesis, characterization, toxicity and antibacterial effect of TiO2 nanoparticles obtained mainly through biogenic processes. The importance of biogenic synthesized nanoparticles has been increasing in recent years; however, more studies aimed at better characterizing the potent toxicity of these nanoparticles are still necessary for nanosafely considerations and environmental perspectives. A wide array of inorganic nanoparticles has been synthesized by using biogenic enzymatic methods and their applications in many cutting-edge technological areas have been explored. In the present work, Titanium dioxide nanoparticles were synthesized by using the culture supernatant of Lactobacillus sp. that was obtained from yoghurt. Lactobacilli have a negative electro kinetic potential, which is suitable for the attraction of cations, a step that is required for the biosynthesis of metallic nanoparticles. Ultrafine size and morphological properties of synthesized nanoparticles was determined by TEM, SEM and X- ray diffraction where the size of Nanoparticles were 5-20nm, found morphologically as aggregate form. The antibacterial activity of the biosynthesized TiO2 nanoparticles was tested against Staphylococcus aureus and Escherichia coli. Maximum zone of inhibition was observed against Escherichia coli. Thus the biogenic TiO2 nanoparticles can be explored in biomedical and nanotechnology applications without any adverse side effects.

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