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Bioremoval of triphenylmethane dye malachite green by pseudomonas stanieri: kinetic and equilibrium studies

Author: 
Ilayaraja, M., Kulandaivel, S. and Sayee Kannan, R.
Subject Area: 
Physical Sciences and Engineering
Abstract: 

An isolated marine bacterium Pseudomonas stanieri was studied for its biodecolorization potential using Malachite Green (MG) as a model dye pollutant. Various carbon sources (Sucrose and Starch) and nitrogen source (peptone) were optimized for bacterial growth and decolorization of dye. Maximum rate of MG decolorization (50 ppm) was observed within 60 h by the isolated bacterium in the culture medium by plate assay method. The decolorization was confirmed by UV-VIS spectrophotometer. The initial dye solution showed high peak at the wavelength of 619 nm. The decolorization dye showed disappearance of peak. The decolorization rate was in direct proportion to the biomass concentration. The effect of dye concentration (50-250 ppm), pH (6-9), temperature (30 -50˚C) and inoculums size (5-25 ml) on dye removal potential of the bacterium was studied. Kinetic data were described by pseudo-first and second-order models. Langmuir and Freundlich adsorption models were applied to analyze the isotherms and isotherms constant.

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