In the present investigation the antimicrobial activities of some traditional medicinal plants which were selected based on medicinal reports practiced by native people of Myeik Township, Tanintharyi region in Myanmar. Antimicrobial activities of aqueous extract of Lawsonia inermis L.(Henna), Oroxylum indium (L.)Vent (midnight horror) and Melastoma melabathricum L.(Malabar ) tested against the following microorganism like Staphylococcus aureus, Bacillus pumilus ,Bacillus subtilis , Pseudomonas aeruginosa ,Escherichia coli and Candida albicans by Agar-well diffusion method. The Lawsonia inermis L. showed maximum zone of inhibition against Staphylococcus aureus, (15 mm) and minimum (13 mm) of Candida albicans. The Oroxylum indium (L.)Vent showed maximum zone of inhibition against Staphylococcus aureus, (13 mm) and minimum (11 mm )of Pseudomonas aeruginosa. Melastoma melabathricum L. showed maximum zone of inhibition against Bacillus subtilis (13 mm) and the minimum (12 mm) of Candida albicans. The results revealed the presence of important medicinal phytochemicals constituents, such as alkaloids, flavonoids, phenols, saponins, α-amino acids, reducing sugar, phlobatannins and tannins. were present in the Lawsonia inermis L. and Melastoma melabathricum L.For Oroxylum indium (L.)Vent, presence of alkaloids, glycosides, phlobatannins, phenolic compounds, α-amino acids, saponins and tannins which interpret its medicinal values. Among the three samples, coumarins was found to be presence in Lawsonia inermis L.
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