CERTIFICATE

IMPACT FACTOR 2021

Subject Area

  • Life Sciences / Biology
  • Architecture / Building Management
  • Asian Studies
  • Business & Management
  • Chemistry
  • Computer Science
  • Economics & Finance
  • Engineering / Acoustics
  • Environmental Science
  • Agricultural Sciences
  • Pharmaceutical Sciences
  • General Sciences
  • Materials Science
  • Mathematics
  • Medicine
  • Nanotechnology & Nanoscience
  • Nonlinear Science
  • Chaos & Dynamical Systems
  • Physics
  • Social Sciences & Humanities

Why Us? >>

  • Open Access
  • Peer Reviewed
  • Rapid Publication
  • Life time hosting
  • Free promotion service
  • Free indexing service
  • More citations
  • Search engine friendly

Comparative studies between the mother plant and the micropropagated plantlets of black berry (Rubus fruticosus L.)

Author: 
Mohamed M. AbdAlla, Nahed A.K. Rashed and Mohamed Abd Elsalam Yosef
Subject Area: 
Life Sciences
Abstract: 

Blackberry (Rubus fruticosus L.) is a shrub with great importance because of its fruits and /or aerial parts have a wide use in pharmaceutal, medicinal and industrial healthcare purposes, in-addition to their nutritional value. In the present study, inter simple sequence repeats (ISSR) and sequence-related amplified polymorphism(SRAP) analyses were used to evaluate genetic stability of Rubus fruticosus L.̔̔ Triple Crown̕ micropropagated plantlets and compare or detect possibly existing genetic variation between them and their donor mother plant. Introduced shoot tips of Rubus fruticosus L.̔̔ Triple Crown̕ were micropropagated in North Sinai Research Station, Desert Research Center. Sterilized excised shoot tips of them were cultured on MS basal salt medium supplemented with benzyl adenine 0.5 mg/L. Then developed shoots successfully transferred to multiplication media for several subcultures and subsequently to rooting medium. Genomic DNA of five micropropagated plantlets (samples) that were phenotypically normalregenerates and essentially identical with their mother plant, in-addition to their donor (mother plant) was extracted using modified CTAB method. Ten ISSR primers and twenty three different SRAP primer combinations were used for this study. Comparisons of each ISSRand SRAPbanding patters average ofmean percentage of similarity which calculated by Nei & Li similarity coefficient and dendrogams constructed based on the UPGMA clustering method showed that the micropropagated plantlets (samples) exhibited somaclonal variation and not true-to-type. Also, polymorphic information content and marker index values for the two markers indicated the presence of polymorphism between the studied samples and their mother plant. In-addition, the discriminating capacity and efficiency of ISSR and SRAP markers for genetic analyses were high, but SRAP markers had better capacity for comparative study and quantifying genetic diversity between Rubus fruticosus L. ̔Triple Crown̕ micropropagated plantlets (samples) and their mother plant.

PDF file: 

ONLINE PAYPAL PAYMENT

IJMCE RECOMMENDATION

Advantages of IJCR

  • Rapid Publishing
  • Professional publishing practices
  • Indexing in leading database
  • High level of citation
  • High Qualitiy reader base
  • High level author suport

Plagiarism Detection

IJCR is following an instant policy on rejection those received papers with plagiarism rate of more than 20%. So, All of authors and contributors must check their papers before submission to making assurance of following our anti-plagiarism policies.

 

EDITORIAL BOARD

Dr. Swamy KRM
India
Dr. Abdul Hannan A.M.S
Saudi Arabia.
Luai Farhan Zghair
Iraq
Hasan Ali Abed Al-Zu’bi
Jordanian
Fredrick OJIJA
Tanzanian
Firuza M. Tursunkhodjaeva
Uzbekistan
Faraz Ahmed Farooqi
Saudi Arabia
Eric Randy Reyes Politud
Philippines
Elsadig Gasoom FadelAlla Elbashir
Sudan
Eapen, Asha Sarah
United State
Dr.Arun Kumar A
India
Dr. Zafar Iqbal
Pakistan
Dr. SHAHERA S.PATEL
India
Dr. Ruchika Khanna
India
Dr. Recep TAS
Turkey
Dr. Rasha Ali Eldeeb
Egypt
Dr. Pralhad Kanhaiyalal Rahangdale
India
DR. PATRICK D. CERNA
Philippines
Dr. Nicolas Padilla- Raygoza
Mexico
Dr. Mustafa Y. G. Younis
Libiya
Dr. Muhammad shoaib Ahmedani
Saudi Arabia
DR. MUHAMMAD ISMAIL MOHMAND
United State
DR. MAHESH SHIVAJI CHAVAN
India
DR. M. ARUNA
India
Dr. Lim Gee Nee
Malaysia
Dr. Jatinder Pal Singh Chawla
India
DR. IRAM BOKHARI
Pakistan
Dr. FARHAT NAZ RAHMAN
Pakistan
Dr. Devendra kumar Gupta
India
Dr. ASHWANI KUMAR DUBEY
India
Dr. Ali Seidi
Iran
Dr. Achmad Choerudin
Indonesia
Dr Ashok Kumar Verma
India
Thi Mong Diep NGUYEN
France
Dr. Muhammad Akram
Pakistan
Dr. Imran Azad
Oman
Dr. Meenakshi Malik
India
Aseel Hadi Hamzah
Iraq
Anam Bhatti
Malaysia
Md. Amir Hossain
Bangladesh
Ahmet İPEKÇİ
Turkey
Mirzadi Gohari
Iran