The present study investigates comparative study on biodegrdation of azo dyes by Klebsiella pneumoniae isolated from environmental and clinical samples. The samples were collected from environmental such as water contaminated with dyes and clinical samples such as throat, pus and urine samples Klebsiella pneumoniae. The azo dye used in this study namely Congo red, Methyl red, Methyl orange, Eriochrome black T. K. pneumoniae isolated from clinical samples degrades higher percentage of Methyl red 88.76% and Congo red 66.45% and least percentage of Methyl orange 7.00%. Whereas the degradation of azo dye by K. pneumoniae isolated from water of textile dye effluent, higher percentage of degradation was found against Methyl orange 95.45% Congo red 76.38% Eriochrome Black T 73.18% and least degradation against Methyl red 8.86%. By comparing both samples for the degradation, environmental sample like water was best decolorizer of azo dye compared to clinical samples.
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