The eight values of organizational culture namely (openness, confrontation, trust, authenticity, pro-action, autonomy, collaboration and experimentation) were analyzed through t-test. The purpose of the present research work is to compare culture of universities of Rajasthan i.e. top and middle management. Respondents were directly contacted for filling up the standard questionnaire of OCTAPACE-Profile, developed by Dr. Udai Pareek. The results conclude that there is significant difference on openness, confrontation, trust, authenticity, pro-action, collaboration and experimentation dimensions of top and middle management. The significance of the study is based on the challenges facing higher education and to improve their academic standard through organizational culture of top and middle level management.