Objective: This study was designed to compare between POCT glucometer and Laboratory reference method for glucose measurement in neonates especially in hypoglycemic conditions. It also evaluated the acceptability of POCT glucometer to measure blood glucose concentration in neonatal hypoglycemia. Material and Methods: 163 samples were collected, only 70 samples were included in this study. The cut off for glucose concentration <40mg/dL was taken for neonatal hypoglycemia since there is no specific demarcation exists in hypoglycemia of neonates., Glucose dehydrogenase (Accu-Chek Aviva) and Hexokinase method (Reference Laboratory method) was used to measure glucose measurement in samples that were obtained. Results: Glucometer accuracy was evaluated using linear regression, Passing-Bablock regression, Bland-Altman analysis. There was no significant difference. Clarke Error Grid analysis, >98% results were in zone A. The Mean bias of Accu Chek Aviva was 2.3%; with P <0.003 Conclusion: POCT Accu-chek Aviva glucose measurement performance was acceptable in hypoglycemic range.