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Comparison of davidson bodies with barr bodies

Author: 
Shivaji Selvi, Harikrishnan Prasad, Muthusamy Rajmohan, K.K. Sri Chinthu, Viswanathan Prema and Loganathan Mahalakshmi
Subject Area: 
Health Sciences
Abstract: 

Objective: To compare the accuracy of gender determination using two different techniques - Davidson bodies identification in blood smear and Barr bodies identification in buccal smear. Methods: A blinded study using 30 subjects (15 males and 15 females) was performed. Peripheral blood smear was obtained from each patient and stained with Leishman stain for identification of Davidson bodies. Buccal smears were obtained from each patient and stained with H and E stain for identification of Barr bodies. 100 neutrophils in blood smear and 100 squamous cells in buccal smear were identified and examined for the presence of Davidson bodies and Barr bodies respectively. Result: Our results showed that females had 1-2% of Davidson bodies in neutrophils compared to 0% in males. However, such clear cut differentiation was not obtained using Barr bodies. Conclusion: Davidson bodies and Barr bodies are independent variables. Davidson bodies in blood smear are highly specific when compared to Barr bodies in buccal smear. Thus morphological gender determination using Davidson bodies in hematopathology is easy, reliable, less time consuming and cost effective.

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