Thyroid dysfunction is an endocrine disorder commonly associated with Type-II Diabetes mellitus though their correlation is yet to be clearly understood. This study was conducted to establish a correlation between blood glucose level and thyroid profile in type-II Diabetic Mellitus patients. The study involved two groups: Case group and Control group. 50 patients of type-II Diabetes Mellitus having high circulating levels of Thyroid stimulating hormone (TSH) were considered as Case group and 50 non diabetic, healthy individuals having normal thyroid level were taken as the Control group. The study was conducted at Gujarat Cancer Society Medical College, Hospital and Research Center, Ahmedabad. Serum Glucose and thyroid hormones- thyroxine and triiodothyronine (Total T4 and T3) and thyroid stimulating hormone (TSH) were measured. Determination of serum hormones concentration was carried out using highly sensitive RIA technique. The results indicated a strong interrelation between recognition and treatment of thyroid dysfunction and management of Type-II Diabetes mellitus patients as this condition affects glycemic control.