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Curcumin suppresses endosulfan toxicity in rabbit’s cornea

Author: 
Eman Mohamed Aly and Eman saad Elabrak
Subject Area: 
Life Sciences
Abstract: 

The aim of the present study was to evaluate the change in corneal protein and oxidative stress state after endosulfan-administered in rabbits and the effect of topical curcumin. The treatment scheme for the different groups was as follows: control group, endosulfan group, Endosulfan+40 μmol/L curcumin, endosulfan+ curcumin 80μmol/L and Endosulfan + curcumin 160 μmol/L. All animals groups decapitated after 6 weeks then eyes were inoculated and corneas were isolated for determination of protein content, SDS-PAGE, catalase (CAT), superoxide dismutase (SOD),Na+-K+-ATPase, and the malondialdehyde (MDA) level activities were measured in the cornea. The results indicated that for endosulfan group a significant increase (p < 0.05) in total protein content accompanied by shift of the corneal protein peaks towards high molecular weight. Also a significant increase (p < 0.05) in catalase and MDA activities was observed. Topical application of curcumin was useful in reducing oxidative stress induced by endosulfan.

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