CALL FOR PAPERS

CERTIFICATE

IMPACT FACTOR 2019

Subject Area

  • Life Sciences / Biology
  • Architecture / Building Management
  • Asian Studies
  • Business & Management
  • Chemistry
  • Computer Science
  • Economics & Finance
  • Engineering / Acoustics
  • Environmental Science
  • Agricultural Sciences
  • Pharmaceutical Sciences
  • General Sciences
  • Materials Science
  • Mathematics
  • Medicine
  • Nanotechnology & Nanoscience
  • Nonlinear Science
  • Chaos & Dynamical Systems
  • Physics
  • Social Sciences & Humanities

Why Us? >>

  • Open Access
  • Peer Reviewed
  • Rapid Publication
  • Life time hosting
  • Free promotion service
  • Free indexing service
  • More citations
  • Search engine friendly

Plagiarism Detection

IJCR is following an instant policy on rejection those received papers with plagiarism rate of more than 20%. So, All of authors and contributors must check their papers before submission to making assurance of following our anti-plagiarism policies.

 

 

 

 

 

 

 

 

 

 

 

 

Detection of bovine rotavirus in neonatal calf diarrhoea by elisa, fat and transmission electron Microscopy

Author: 
Suresh T., Rai R. B., Wani M. Y., Damodaran, T. and Dhama, K.
Subject Area: 
Health Sciences
Abstract: 

The present study was designed to investigate and compare the sensitivity of enzyme linked immunosorbent assay (ELISA), fluorescent antibody test (FAT) and transmission electron microscopy (TEM) for the detection of bovine rotavirus during neonatal calf diarrhea. A total of 112 faecal samples of diarrhoeic calves below three months were collected from organized dairy farms of Namakkal (Tamil Nadu) and Bareilly (Uttar Pradesh), India. Out of these, 27 faecal samples (24.10%) were found positive for rotavirus by ELISA and TEM. The percent positivity ranged from 18.86 % to 55.55% in different organized farms. The rotavirus samples when subjected to isolation in MDBK cells resulted in successful isolation of bovine group A rotavirus in twenty samples. The characteristic cytopathic effects (CPE) were observed from second passage onwards and comprised of clumping and rounding of infected cells, detachment of monolayer, intracytoplasmic eosinophilic inclusions, syncytia and leaving empty vacuole space in MDBK cell line. Rotavirus antigen in cell culture adapted virus was detected by direct fluorescent antibody test (dFAT). The sensitivity and specificity of ELISA and TEM were 100% and 92.32% respectively, considering virus isolation followed by dFAT as standard test. Study indicates that ELISA is most suitable and sufficient test for routine diagnosis of bovine rotaviral as compared to laborious TEM analysis for diarrheic samples; however, isolation of the virus in cell culture remains indispensable for the identification of doubtful specimens. To our knowledge, this is the first report from India employing TEM, ELISA and cell culture based FAT for detection and comparison of these tests for bovine rotavirus group A from diarrhoeic calves.

PDF file: 

IJMCE RECOMMENDATION

ONLINE PAYPAL PAYMENT

CURRENT ISSUE

NEWS

CHIEF EDITOR
Rosane Cavalcante Fragoso, Brasil
ASSOCIATE CHIEF EDITOR

   

Jean-Marc SABATIER
Chief Scientific Officer and Head of a Research Group
France

Advantages of IJCR

  • Rapid Publishing
  • Professional publishing practices
  • Indexing in leading database
  • High level of citation
  • High Qualitiy reader base
  • High level author suport

 

 

 

 

 

 

 

 

 

 

 

EDITORIAL BOARD

Luai Farhan Zghair
Iraq
Hasan Ali Abed Al-Zu’bi
Jordanian
Fredrick OJIJA
Tanzanian
Firuza M. Tursunkhodjaeva
Uzbekistan
Faraz Ahmed Farooqi
Saudi Arabia
Eric Randy Reyes Politud
Philippines
Elsadig Gasoom FadelAlla Elbashir
Sudan
Eapen, Asha Sarah
United State
Dr.Arun Kumar A
India
Dr. Zafar Iqbal
Pakistan
Dr. SHAHERA S.PATEL
India
Dr. Ruchika Khanna
India
Dr. Recep TAS
Turkey
Dr. Rasha Ali Eldeeb
Egypt
Dr. Pralhad Kanhaiyalal Rahangdale
India
DR. PATRICK D. CERNA
Philippines
Dr. Nicolas Padilla- Raygoza
Mexico
Dr. Mustafa Y. G. Younis
Libiya
Dr. Muhammad shoaib Ahmedani
Saudi Arabia
DR. MUHAMMAD ISMAIL MOHMAND
United State
DR. MAHESH SHIVAJI CHAVAN
India
DR. M. ARUNA
India
Dr. Lim Gee Nee
Malaysia
Dr. Jatinder Pal Singh Chawla
India
DR. IRAM BOKHARI
Pakistan
Dr. FARHAT NAZ RAHMAN
Pakistan
Dr. Devendra kumar Gupta
India
Dr. ASHWANI KUMAR DUBEY
India
Dr. Ali Seidi
Iran
Dr. Achmad Choerudin
Indonesia
Dr Ashok Kumar Verma
India
Thi Mong Diep NGUYEN
France
Dr. Muhammad Akram
Pakistan
Dr. Imran Azad
Oman
Dr. Meenakshi Malik
India
Aseel Hadi Hamzah
Iraq
Anam Bhatti
Malaysia
Md. Amir Hossain
Bangladesh
Ahmet İPEKÇİ
Turkey
Mirzadi Gohari
Iran