Gene construct for resistance against tomato leaf curl virus (ToLCV) using replicase (rep) gene sequences of Tomato leaf curl virus from natural host plant was developed. A binary vector carrying the antisense rep gene along with the npt II gene was used for development of truncated rep gene construct. The mechanism of resistance appears RNA-mediated, The concept of pathogen-derived resistance was introduced in plant virology based on the report that transgenic tobacco expressing tobacco mosaic virus (TMV) coat protein showed a resistance to TMV infection This type of resistance generally referred to as coat protein-mediated resistance, has been described for many virus/host systems, but is restricted to the virus closely related to the expression of functional or altered introduced viral replicase gene. Viral gene suppression involves a coordinated series of sub cellular events that ultimately lead to the post-transcriptional silencing of gene expression