The dispersal of fungal spore districts like Thanjavure, Pudukottai and Madurai, Tamil Nadu, India was enumerated during the period 2010-2011 in the present investigation. In all twenty sample sites of Thanjavur district this fluctuations showed some uniformity. In Thanjavure, the lowest concentrations of fungal spores were observed both in June and July. The fluctuation trends in all months for all sampling sites in Pudukkottai district was as similar as observed in Thanjavur district. In Madurai, the highest range of fungal spores observed in the month of April suddenly decreased to 50- 60% in January and these trends continued up to June. Hence, the fungal spore concentration fluctuation trends were more or or less similar in all sampling places of Thanjavur, Pudukkottai and Madurai districts.