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Dissolution method development and validation of enrofloxacin tablets by uv spectrophotometry

Author: 
Manasa, Mahes h, Ibrahim and Hepsebah, N. J. R.
Subject Area: 
Health Sciences
Abstract: 

The main aim of the article is to develop a simple dissolution method for Enrofloxacin immediate release tablets by UV spectroscopy and validate as per ICH guidelines. The optimized dissolution method includes potassium di hydrogen phosphate pH 4.5 as dissolution media, apparatus as USP Type 2 Paddle, rpm as 100, temperature of dissolution media as 37±0.5ºC, dissolution volume as 500ml, dissolution time point as 30 minutes, working concentration of standard and sample as 5μg/ml and a detection wavelength of 276 nm. The developed method resulted in Enrofloxacin exhibiting linearity in the range 1.25-10μg/ml. System precision and intra-day precision are exemplified by relative standard deviation of 0.148% and 0.950% respectively. Method was found to be rugged/inter day precise as % RSD was found to be 0.924%. Percentage Mean recovery was found to be in the range of 90-110 % by absolute method during accuracy studies. Hence it can be concluded that effective dissolution method by UV spectroscopy is developed and validated which can be applicable in various pharmaceutical industries.

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