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Distribution of hepatitis b virus genotypes among chronic Hepatitis B patients in a tertiary care hospital in New Delhi

Author: 
Bibhash Nandi, Anita Chakravarti, Preena Bhalla, and Premashis Kar
Abstract: 

Introduction: Hepatitis B Virus (HBV) genotypes A and D are well documented from different parts of India, while genotype C in addition to mixed genotype A and D (A/D) has been reported and characterized from Eastern India. HBV genotyping is a useful tool for understanding the epidemiology of HBV infection. HBV genotype is not only predictive of clinical outcomes but has also been associated with response to interferon treatment. Material and methods: The HBV genotype was identified by using PCR-RFLP analysis of the S gene in 50 HBsAg positive adult patients. Restriction enzyme digestion was carried out by using AlwI, HphI, NciI, NlaIV and EarI nuclease enzymes to see the different patterns of cleavage that would occur at the specific sites. Results: The most common HBV genotype was D (31/50, 62%), followed by genotype A (15/50, 30%) and mixed A/D genotype (4/50, 8%). Conclusions: These findings suggest that Genotypes A, D and A/D exist in chronic hepatitis B patients in New Delhi, and among these Genotype D is the commonest.

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