Effect of arsenic and manganese on cell division of green gram

In this study, the effects of arsenic and manganese on the cell division in root tips of green gram were conducted. For this purpose, Vigna radiata were germinated into various concentrations of As and Mn (5, 10, 25, 50 100 mg/l) at 23-24°C for 72 h. Chromosome aberration assay was used to determine the mitotic indices and rate of chromosome aberration in green gram root tip cells due to As and Mn treatment. The results showed that the mitotic indices were complicated due to different concentrations of As and Mn. However, the increase in As concentration has led to a gradual increase in the percentage of chromosomal aberration and than manganese on the root tip cells during mitotic index. They exhibited many chromosomal abnormalities such as formation of chromosome bridges, laggards, stickiness, precocious movement, formation of binucleate structure and other abnormal behaviors. There was no considerable change in 2n number of chromosome with the increase in As concentrations. It is concluded that the As and Mn has significant mutagenic effect on the root tip cells of green gram.